One- and two-electron reduction of molybdate reversibly bound to the archaeal tungstate/molybdate transporter WtpA.
نویسندگان
چکیده
Reversible binding of the tetrahedral oxoanions MoO(4)(2-) and WO(4)(2-) to two carboxylato ligands of the soluble scavenger protein WtpA from the hyperthermophilic archaeon Pyrococcus furiosus enforces a quasi-octahedral MO(6) coordination in which the +VI oxidation state is destabilized.
منابع مشابه
Tungsten transport protein A (WtpA) in Pyrococcus furiosus: the first member of a new class of tungstate and molybdate transporters.
A novel tungstate and molybdate binding protein has been discovered from the hyperthermophilic archaeon Pyrococcus furiosus. This tungstate transport protein A (WtpA) is part of a new ABC transporter system selective for tungstate and molybdate. WtpA has very low sequence similarity with the earlier-characterized transport proteins ModA for molybdate and TupA for tungstate. Its structural gene ...
متن کاملClassification of a Haemophilus influenzae ABC transporter HI1470/71 through its cognate molybdate periplasmic binding protein, MolA.
molA (HI1472) from H. influenzae encodes a periplasmic binding protein (PBP) that delivers substrate to the ABC transporter MolB(2)C(2) (formerly HI1470/71). The structures of MolA with molybdate and tungstate in the binding pocket were solved to 1.6 and 1.7 Å resolution, respectively. The MolA-binding protein binds molybdate and tungstate, but not other oxyanions such as sulfate and phosphate,...
متن کاملMolybdenum incorporation in tungsten aldehyde oxidoreductase enzymes from Pyrococcus furiosus.
The hyperthermophilic archaeon Pyrococcus furiosus expresses five aldehyde oxidoreductase (AOR) enzymes, all containing a tungsto-bispterin cofactor. The growth of this organism is fully dependent on the presence of tungsten in the growth medium. Previous studies have suggested that molybdenum is not incorporated in the active site of these enzymes. Application of the radioisotope (99)Mo in met...
متن کاملStructural basis of trans-inhibition in a molybdate/tungstate ABC transporter.
Transport across cellular membranes is an essential process that is catalyzed by diverse membrane transport proteins. The turnover rates of certain transporters are inhibited by their substrates in a process termed trans-inhibition, whose structural basis is poorly understood. We present the crystal structure of a molybdate/tungstate ABC transporter (ModBC) from Methanosarcina acetivorans in a ...
متن کاملAlternative Nitrogenases in Anabaena variabilis: The Role of Molybdate and Vanadate in Nitrogenase Gene
Anabaena variabilis ATCC 29413 has two distinct nitrogenases that function in heterocysts, a conventional Mo-nitrogenase and an alternative V-nitrogenase. Synthesis of these two enzymes was repressed in cells growing with a source of fixed nitrogen, such as ammonium; however, the V-nitrogenase was also repressed by Mo. Expression of the V-nitrogenase which was not affected by V and expression o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Dalton transactions
دوره 39 شماره
صفحات -
تاریخ انتشار 2009